How to choose pipette tips? "The pipette tips that can be installed are the pipette tips that can be used"
——This is almost the general perception of all users on the adaptability of pipette tips. This statement can be said to be partly true but not entirely true.
The pipette tips that can be installed on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here. Answering this question requires data to speak.
1. You may wish to perform a performance test after matching the pipette with the pipette tips. After rinsing the pipette tips, repeat the sample addition operation several times, weigh the sample amount each time, and record the reading.
2. Calculate the accuracy and precision of the pipetting operation after converting the density of the test liquid into volume.
3. we want to choose our pipette tips with good accuracy. If the pipette tips are matched with poor accuracy, it means that the tightness between the pipette tips and the pipette cannot be guaranteed, making the results of each operation irreproducible
So what are the minimum points that good pipette tips should have?
Good pipette tips depend on concentricity, taper, and important point is adsorption;
1. Let's talk about the taper first: if it is better, it will match the gun very well, and the suction will be more accurate;
2. Concentricity: Concentricity is whether the suction nozzle of the pipette tips and the circle where the pipette tips are connected to the pipette are perpendicular to the same circle center. If not, it means that the concentricity is not good;
3. Finally, important one is our adsorption: adsorption is related to the material of pipette tips. If the material of pipette tips is not good, it will affect the accuracy of pipetting, resulting in a large amount of liquid retention or referred to as For hanging on the wall, causing errors in pipetting;
So everyone should pay special attention to the above three points when choosing pipette tips
If the bad pipette tips are placed in a row, the spacing will be different! You will see obvious distortions, which is an important step in choosing whether it is a good pipette tip.